Anabolic Steroid Urine Testing

Over the past decade, anabolic steroid abuse and the use of other performance-enhancing drugs has become a national concern! Androgenic-anabolic steroids and other performance-enhancing drugs are banned by major amateur and professional sports authorities. Individuals of various ages abuse steroids, but it is the abuse among high school students which is a serious problem!


Using steroids without a prescription can cause serious, adverse health effects. Using steroids and other performance-enhancing drugs can give an unfair advantage over the competition, and is cheating. Testing for steroids can help deter their use among school pupils. Steroids are drugs that should only be used to treat medical conditions. Possession, use or dealing of most steroids without a prescription is illegal.

Schools recognizes that it will take a community-wide effort by parents, coaches, pupils, teachers and physicians to attack this growing challenge. Steroid testing is one tool that can assist in discouraging pupils from taking steroids.

We can utilizes a broad range of analytical techniques and instrumentation to meet your demands for urine and blood testing.  Our contracted laboratory’s capabilities include:  immunoassay, gas chromatography mass spectrometry (GC/MS), liquid chromatography mass spectrometry (LC/MS), isotope ratio mass spectrometry (IRMS), gel electrophoresis, and bio and chemiluminescence.  Laboratory instrumentation includes:

We Can Test For: 

Steroid/Sports Drug Testing frequently asked questions


What are prohormones and designer steroids?

The number of available prescription anabolic steroids has not changed since the late 1960’s. However, since 1997 many new AAS have been released into circulation as nutritional supplements. They are commonly known as prohormones and designer steroids.

Why are some steroids on steroid panel grouped together?

Some steroids, when ingested, convert into each other forming an identical set of urinary metabolites. It is impossible to determine which drug in the group was administered.

Why are steroids screened by GC/MS?

Most steroids break down in the body into multiple metabolites that are excreted in urine at low concentrations. These substances are similar to each other and to the endogenous steroids, normally present in urine in higher concentrations. GC/MS screening for the Steroid Test because it provides an efficient separation and specific detection of individual compounds.

The GC/MS confirmation procedure is specific to each individual steroid.

For multiple positive screens, multiple individual confirmations are set up. Specimens that screen positive are also confirmed by GC/MS using a separate aliquot of urine.

What is the T/E ratio?

Testosterone and its precursors may be endogenous (produced in the body naturally) or exogenous (ingested as drugs or supplements). The T/E ratio is used to distinguish between the two. This ratio is a urine concentration ratio of two steroids, testosterone (T) and its natural isomer, epitestosterone (E). The normal average ratio is approximately 1, with individual variation on both sides, either higher or lower. Ingestion of exogenous testosterone or its precursors suppresses internal steroid production in the body. Both endogenous T and E would be suppressed. However, total testosterone concentration in urine will rise above normal due to ingested drug. Low E and high T cause the T/E ratio to rise above 6 (cut-off), indicating testosterone abuse.

How long can steroids be detected in urine?

Detection time may vary from a few days (short-term pill) to as long as 12-18 months (oil-based injection), depending on the pharmacological form used, route of administration, length of usage and quantity. Individual differences may also contribute to the duration of the drug in the body. Test results cannot show when the drug was used, i.e. how long before the urine was collected. 

How stable are steroid metabolites in urine?

Refrigerated or frozen urine shows very little or no steroid metabolite degradation during prolonged (several years) storage.

This premier service is comparable to that currently provided by WADA accredited laboratories supporting doping control programs in sport. Toxicologists with a high level of expertise in athletic drug testing, including extensive experience in the Olympic laboratory.